Core Assays

Cytokeratin (OSCAR) Mouse Monoclonal Primary Antibody is intended for laboratory use in the detection of a broad spectrum of cytokeratin proteins in formalin-fixed, paraffin-embedded human tissue stained in qualitative immunohistochemistry (IHC) testing on BenchMark IHC/ISH instruments.

This antibody stains cytokeratins present in normal and malignant epithelial tissues, making it useful for aiding in the identification of epithelial malignancies.1-4

Benefits of Cytokeratin (OSCAR):

• In vitro diagnostic; Ready to use

• Decreased cross-reactivity with gliomas vs. AE1/AE32

 • Reactive with cytokeratin 18, which can identify tumors missed by AE1/AE3 (e.g. hepatocellular carcinoma)2

References

1 Galera P, et al. Diagnosis of Metaplastic Breast Carcinoma: Keratin OSCAR Versus Other Cytokeratins. Appl Immunohistochem Mol Morphol. 2016 Oct;24(9):622-626

2 Ordóñez NG. Broad-spectrum immunohistochemical epithelial markers: a review. Hum Pathol. 2013 Jul;44(7):1195-215

3 Goddard MJ, et al. Comparison of commercially available cytokeratin antibodies in normal and neoplastic adult epithelial and non-epithelial tissues. J Clin Pathol. 1991 Aug;44(8):660-3.

4 Chu PG, et al. Keratin expression in human tissues and neoplasms. Histopathology. 2002 May;40(5):403-39

The FGFR2b-specific mouse monoclonal antibody (FPR2-D) detects FGFR2 expression in gastric cancer tissues with FGFR2b protein overexpression.1 The VENTANA FGFR2b (FPR2-D)Mouse Monoclonal Antibody is intended for laboratory use in the qualitative immunohistochemical detection of FGFR2b by light microscopy in sections of formalin-fixed, paraffin-embedded tissues stained on a BenchMark IHC/ISH instrument.

References

1 Deshpande AM, Palencia S, Bellovin DI, et al. Abstract 2845: Expression of FGFR2b in gastric cancer as measured by immunohistochemistry with a highly specific monoclonal antibody.

Cancer Research. 2014;74 (19 Supplement):2845-2845. https://www.researchgate.net/publication/276504277_Abstract_2845_Expression_of_FGFR2b_in_gastric_cancer_as_measured_by_

immunohistochemistry_with_a_highly_specific_monoclonal_antibody. Accessed on January 21, 2024.

Immunohistochemical detection of SF-1 may provide value for identifying

sex-cord stromal tumors - including Leydig cell, Sertoli cell and granulosa cell neoplasms - from other tumors.1,2 In addition, SF-1 testing may have utility in distinguishing between primary adrenal cortical lesions and their histologic mimics such as renal cell carcinoma.3

SF-1 (EP434) Rabbit Monoclonal Primary Antibody is intended for laboratory use in the detection of the steroidogenic factor-1 protein in formalin-fixed, paraffin-embedded tissue stained in qualitative immunohistochemistry on BenchMark IHC/ISH instruments.

Benefits of SF-1 (EP434):

• In vitro diagnostic; Ready to use

• Identification and differentiation of sex-cord stromal tumors (Leydig cell, Sertoli cell and granulosa cell neoplasms) from other tumors.1,2

• Useful in distinguishing between primary adrenal cortical lesions and their histologic mimics such as renal cell carcinoma.3

References

1 Sangoi AR et al. Evaluation of SF-1 Expression in Testicular Germ Cell Tumors: A Tissue Microarray Study of 127 cases. Applied Immunohistochemistry and Molecular Morphology 2013 21(4): 318-321

2 Zhao, C et al. Identification of the Most Sensitive and Robust Immunohistochemical Markers in Different Categories of Ovarian Sex Cord-stromal Tumors. American Journal of Surgical Pathology 2009 33(3): 354-366

3 Sangoi, AR et al. Immunohistochemical distinction of primary adrenal cortical lesions from metastatic clear cell renal cell carcinoma: a study of 248 cases. American Journal of Surgical Pathology 2011 35(5): 678-686

CD8 is a transmembrane glycoprotein expressed by cytotoxic T-cells and at low levels in NK-cells and a subpopulation of bone marrow cells1-3. A cytotoxic T-cell marker, CD8 positive T-cells are mainly involved in cytotoxic immunoreactions4. CD8 is absent on immature T-cells and expressed in later stages of T-cell development as the cell matures into a cytotoxic T-cell5-6. CD8 (SP239) Rabbit Monoclonal Primary Antibody is intended for laboratory use in the qualitative immunohistochemical detection of CD8 by light microscopy in sections of formalin-fixed, paraffin-embedded tissue stained on a BenchMark IHC/ISH instrument.

Benefits of CD8 (SP239) antibody:

• In vitro diagnostic; Ready to use

• Recombinant technology offering consistent and reproducible results

• In a comparison antibody test by NordiQC, CD8 (SP239) antibody scored “optimal” and equivalent to the NordiQC reference antibody7

• A cornerstone product in the Roche hematopathology tissue menu, a comprehensive offering of over 70 robust IHC and ISH assays supported by innovative detection, automation and workflow solutions

References

1 Dabbs DJ. Diagnostic Immunohistochemistry Theranostic and Genomic Applications, 5th edition. Vol 5. Amsterdam, Netherlands: Elsevier; 2019.

2 Higgins RA, Blankenship JE, Kinney MC. Application of immunohistochemistry in the diagnosis of non-Hodgkin and Hodgkin lymphoma. Arch Pathol Lab Med. 2008; 132(3):441-461.

3 Naeim F. Principles of Immunophenotyping. In Naeim F, Rao PN, Grody WW, eds. Hematopathology: Morphology, Immunophenotype, Cytogenetics, and Molecular Approaches. Cambridge, MA: Academic Press; 2009.

4 https://www.nordiqc.org/epitope.php?id=22. Accessed 19 April 2023.

5 Rich R, Fleisher T, Shearer W, Frew A, Weyand C. Clinical Immunology Principles and Practice, 5th edition. Vol 5. Amsterdam, Netherlands: Elsevier, 2018.

6 Swerdlow SH, Campo E, Harris NL, et al. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues, 4th edition. Vol 4. Lyon, France: International Agency for Research on Cancer; 2008.

7 Data on file